Gibberellins in Suspensors of Phaseolus coccineus L. Seeds

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Gibberellins in Embryo-Suspensor of Phaseolus coccineus Seeds at the Heart Stage of Embryo Development.

Gibberellins (GAs) in suspensors and embryos of Phaseolus coccineus seeds at the heart stage of embryo development were analyzed by combined gas chromatography-mass spectrometry (GC-MS). From the suspensor four C(19)-GAs, GA(1), GA(4), GA(5), GA(6), and one C(20) GA, GA(44), were identified. From the embryo, five C(19)-GAs GA(1), GA(4), GA(5), GA(6), GA(60) and two C(20) GAs, GA(19) and GA(44) ...

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Gibberellins in Embryo - Suspensor of Phaseolus coccineus Seeds at the Heart Stage of Embryo Development 1

Gibberellins (GAs) in suspensors and embryos of Phaseolus coccineus seeds at the heart stage of embryo development were analyzed by combined gas chromatography-mass spectrometry (GC-MS). From the suspensor four C,9-GAs, GA1, GA4, GA5, GA6, and one C2o GA, GA44, were identified. From the embryo, five C1gGAs GA,, GA4, GAs, GAs, GAo0 and two C20 GAs, GA1,9 and GA4 were identified. The data, in rel...

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Pattern of Variations in Abscisic Acid Content in Suspensors, Embryos, and Integuments of Developing Phaseolus coccineus Seeds.

Free abscisic acid (ABA) content in suspensors, embryos, and integuments was determined during seed development of Phaseolus coccineus. A highly specific and sensitive solid-phase radioimmunoassay based on a monocional antibody raised against free (S)-ABA was used for ABA quantification. Very small amounts of ABA were detected in the suspensor during initial stages of development; later two pea...

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Expression of two genes encoding gibberellin 2- and 3-oxidases in developing seeds of Phaseolus coccineus.

We have isolated PcGA3ox1, a cDNA clone from developing runner bean (Phaseolus coccineus) seeds that shows significant amino acid homology with the gibberellin (GA) 3-oxidases. A recombinant fusion protein of PcGA3ox1 converted GA20 and GA9 to GA1 and GA4, respectively. In situ hybridization results showed that transcripts of this gene accumulate specifically within the suspensor of globular-st...

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Abscisic Acid Accumulation in Developing Seeds of Phaseolus vulgaris L.

Free and bound abscisic acid (ABA) in the pod, seed coat, and embryo were determined separately throughout seed development of Phaseolus vulgaris L. cv. ;Taylor's Horticultural.' An internal standard method of gas-liquid chromatography was used for ABA quantification. In the embryo, two peaks of free ABA occurred at days 22 (1.18 micrograms per gram or 5.5 micromolar) and 28 (1.74 micrograms pe...

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ژورنال

عنوان ژورنال: Plant Physiology

سال: 1986

ISSN: 0032-0889,1532-2548

DOI: 10.1104/pp.82.1.298